A pilot study evaluating the Calibrated Automated Thrombogram assay and application of plasma-thromboelastography for detection of hemostatic aberrations in horses with gastrointestinal disease
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- A pilot study evaluating the Calibrated Automated Thrombogram assay and application of plasma-thromboelastography for detection of hemostatic aberrations in horses with gastrointestinal disease
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Background: Critically ill horses, such as horses with gastrointestinal (GI) disease, often suffer from hemostatic aberrations. Global hemostatic tests examining the initiation of coagulation, clot strength and fibrinolysis, such as the Calibrated Automated Thrombogram (CAT) and plasma-thromboelastography (TEG) have not been evaluated in horses. This study aimed to evaluate CAT and apply plasma-TEG in horses. Test performance of CAT was evaluated on equine platelet poor plasma with intra- and inter-assay variability (CV) and a heparin dilution curve. To examine clinical performance of both tests, group comparisons were assessed comparing healthy horses, horses with mild and severe GI disease with both CAT and plasma-TEG. Results: For CAT, intra- and inter-assay CVs were established for lag-time (1.7, 4.7%), endogenous thrombin potential (1.6, 4.6%), peak (2.6, 3.9%) and time to peak (ttPeak) (1.9, 3.4%). Increasing heparin concentrations led to the expected decrease in thrombin generation. In the group comparison analysis, CAT showed significant higher peak (p = 0.04) and ttPeak (p = 0.008) in the severe GI disease group compared to horses with mild GI disease and healthy horses, respectively. Plasma-TEG showed an increased angle (p = 0.032), maximum amplitude (p = 0.017) and shear elastic force (G) (p = 0.017) in the severe GI disease group compared to healthy horses. Conclusions: CAT performed well in horses. Both CAT and plasma-TEG identified hemostatic aberrations in horses with severe GI disease compared to healthy horses. Further studies including more horses, are needed to fully appreciate the use of CAT and plasma-TEG in this species.
Originalsprog | Engelsk |
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Artikelnummer | 346 |
Tidsskrift | BMC Veterinary Research |
Vol/bind | 17 |
Antal sider | 11 |
ISSN | 1746-6148 |
DOI | |
Status | Udgivet - 2021 |
Bibliografisk note
Funding Information:
The authors are grateful to medical laboratory technologist Louise Bochsen and to laboratory technician Tina Roust from the Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences (SUND), University of Copenhagen, Denmark for their laboratory assistance during this study and to the horse owners who kindly enrolled their horses in the study. The authors would also like to thank Associate professor Dr. Rebecca Langhorn, University of Copenhagen, Denmark for language editing and the Danish Horse Levy Foundation for funding the project.
Funding Information:
The authors would also like to thank Associate professor Dr. Rebecca Langhorn, University of Copenhagen, Denmark for language editing and the Danish Horse Levy Foundation for funding the project.
Publisher Copyright:
© 2021, The Author(s).
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