TY - JOUR

T1 - Effects of experimental mechanical manipulations on local inflammation in the jejunum of horses

AU - Hopster-Iversen, Charlotte C S

AU - Hopster, Klaus

AU - Staszyk, Carsten

AU - Rohn, Karl

AU - Freeman, David E

AU - Rötting, Anna K

PY - 2014/4

Y1 - 2014/4

N2 - OBJECTIVE: To determine characteristics of the inflammatory reaction in the jejunum of horses in response to various mechanical manipulations.ANIMALS: 12 adult warm blood horses without gastrointestinal tract disorders.PROCEDURES: The proximal aspect of the jejunum in each horse was divided into 5 segments, and the following manipulations were performed: manual emptying, placement of Doyen forceps, enterotomy alone, enterotomy with mucosal abrasion, and serosal abrasion. Jejunum samples were collected before (control), immediately after, and 30 minutes after the end of manipulations and histologically evaluated to determine distribution of neutrophils and eosinophils.RESULTS: Macroscopically, all manipulations resulted in jejunal hemorrhage and edema. Compared with control samples, neutrophil numbers were significantly higher after manipulations in the serosa (after all manipulation types), circular muscle layer (after manual emptying), submucosa (after placement of Doyen forceps), and mucosa (after all manipulations except enterotomy alone). Eosinophil numbers were significantly higher in the submucosa after mechanical abrasion of the serosa and manual emptying versus control samples.CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated mechanical manipulation of the jejunum resulted in local inflammatory reactions characterized predominantly by infiltration of neutrophils. This could contribute to the development of postoperative ileus or adhesions in horses without macroscopically detectable injury of the jejunum during surgery.

AB - OBJECTIVE: To determine characteristics of the inflammatory reaction in the jejunum of horses in response to various mechanical manipulations.ANIMALS: 12 adult warm blood horses without gastrointestinal tract disorders.PROCEDURES: The proximal aspect of the jejunum in each horse was divided into 5 segments, and the following manipulations were performed: manual emptying, placement of Doyen forceps, enterotomy alone, enterotomy with mucosal abrasion, and serosal abrasion. Jejunum samples were collected before (control), immediately after, and 30 minutes after the end of manipulations and histologically evaluated to determine distribution of neutrophils and eosinophils.RESULTS: Macroscopically, all manipulations resulted in jejunal hemorrhage and edema. Compared with control samples, neutrophil numbers were significantly higher after manipulations in the serosa (after all manipulation types), circular muscle layer (after manual emptying), submucosa (after placement of Doyen forceps), and mucosa (after all manipulations except enterotomy alone). Eosinophil numbers were significantly higher in the submucosa after mechanical abrasion of the serosa and manual emptying versus control samples.CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated mechanical manipulation of the jejunum resulted in local inflammatory reactions characterized predominantly by infiltration of neutrophils. This could contribute to the development of postoperative ileus or adhesions in horses without macroscopically detectable injury of the jejunum during surgery.

KW - Animals

KW - Digestive System Surgical Procedures/adverse effects

KW - Enteritis/etiology

KW - Horse Diseases/etiology

KW - Horses

KW - Jejunal Diseases/etiology

KW - Jejunum/pathology

U2 - 10.2460/ajvr.75.4.385

DO - 10.2460/ajvr.75.4.385

M3 - Journal article

C2 - 24669925

VL - 75

SP - 385

EP - 391

JO - American Journal of Veterinary Research

JF - American Journal of Veterinary Research

SN - 0002-9645

IS - 4

ER -